IJSSER

Title: INVESTIGATING THE COMBINED EFFECTS OF HEAVY METALS COPPER AND ZINC ON OXIDATIVE STRESS LEVELS OF LYMNAEA STAGNALIS
Authors: Saanvi Vemareddy
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Saanvi Vemareddy Academies of Loudoun, USA
MLA 8 Vemareddy, Saanvi. "INVESTIGATING THE COMBINED EFFECTS OF HEAVY METALS COPPER AND ZINC ON OXIDATIVE STRESS LEVELS OF LYMNAEA STAGNALIS." Int. j. of Social Science and Economic Research, vol. 8, no. 10, Oct. 2023, pp. 3321-3332, doi.org/10.46609/IJSSER.2023.v08i10.022. Accessed Oct. 2023. APA 6 Vemareddy, S. (2023, October). INVESTIGATING THE COMBINED EFFECTS OF HEAVY METALS COPPER AND ZINC ON OXIDATIVE STRESS LEVELS OF LYMNAEA STAGNALIS. Int. j. of Social Science and Economic Research, 8(10), 3321-3332. Retrieved from https://doi.org/10.46609/IJSSER.2023.v08i10.022 Chicago Vemareddy, Saanvi. "INVESTIGATING THE COMBINED EFFECTS OF HEAVY METALS COPPER AND ZINC ON OXIDATIVE STRESS LEVELS OF LYMNAEA STAGNALIS." Int. j. of Social Science and Economic Research 8, no. 10 (October 2023), 3321-3332. Accessed October, 2023. https://doi.org/10.46609/IJSSER.2023.v08i10.022.
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ABSTRACT: Biodiversity, the variety of life within a particular ecosystem or habitat, has been a growing concern in freshwater ecosystems. Streams become more polluted as a result of industrial and agricultural runoff, specifically through heavy metals found in industrial waste as well as pesticides. The impact of pollution on stream health could be studied through bioindicator macroinvertebrates, as their health and mortality reflects the quality of their environment. If an a bioindicator organism’s environment were in poor health conditions, it would reflect on the organism by creating health issues and defects. This study aims to determine the combined effects of heavy metals Copper sulfate and Zinc nitrate on stream health through investigating the oxidative stress levels of macroinvertebrates Lymnaea stagnalis. This involved exposure of Lymnaea stagnalis to the following experimental groups: 15 g/L Zinc nitrate, 15 ug/L Copper sulfate, and regular pond water that will be created in the lab. The disparity between oxidative stress levels of each group would be evaluated. This would be done through a thiobarbituric acid reactive substances assay, which measures the creation of lipid peroxidation products that result from oxidative stress. The creation of lipid peroxidation products indicates oxidative stress through producing a pink color, which will be measured through aspectrophotometer. Additional work is needed to corroborate preliminary results and draw final conclusions. This study may be able to support the creation of new water treatment methods through evaluating the extent to which heavy metals play a significant role in water pollution.

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